Gcn5: The quintessential histone acetyltransferase

Spt7 belongs to the suppressor of Ty (SPT) module Spt-Ada-Gcn5-acetyltransferase (SAGA) complex and is known as yeast ortholog human STAF65γ. lacks intrinsic enzymatic activity but responsible for integrity proper assembly SAGA complex. Here, we determined role subunit in cellular aging. We found that was indispensable a normal lifespan both dividing nondividing cells. In quiescent state cells, required control overall mRNA levels. mitotically active deletion SPT had little effect on recombination rate within heterochromatic ribosomal DNA (rDNA) loci, loss profoundly elevated plasmid-based frequency. Consistently, increased spontaneous Rad52 foci by approximately two-fold upon entry into S phase. These results provide evidence contributes regulation replicative (RLS) chronological (CLS), possibly controlling expression. propose might be involved conserved regulatory pathway eukaryotes.